Method validation for the determination of Chloramphenicol, a banned veterinary antibiotic in shrimps, by using liquid chromatography-tandem mass spectroscopy

Abstract

Chloramphenicol is one of the most widely used antibiotics for both food and domestic animals. It is a broad spectrum antibiotic which interferes with protein the negative polarity of multiple reaction monitoring (MRM) mode. The mass spectrum of the precursor ion was found at m/z =320.9 and product ions were obtained synthesis of many Gram-negative and Gram-positive bacteria and has toxic effects on human.